coli is a diverse species regarding the genetic content and phenotypic and pathogenic traits. coli (UPEC) arises from the distal gut microbiota 1- 3In addition, E. There is a well established belief that uropathogenic E. Keywords: Fecal Escherichia coli, Major virulence factors, Multiplex PCRĮscherichia coli represents as the most encountered etiology for urinary tract infections (UTIs) identified in the gastrointestinal tract of mammals. coli isolates collected from human feces in Iran. This is the first report of the frequency of virulence factors among E. Our findings propose possibility of human feces serving as a source for pathogenic organisms, supporting the notion that fecal materials of humans play a role in the epidemiological chain of extra-intestinal pathogenic E. According to the diversity of different virulence genes, the examined isolates exhibited 29 different patterns.Ĭonclusion: Our results demonstrated that most of the assessed isolates harbored several virulence factors.
Results: The prevalence of virulence genes was as follows: adhesins ( fimH, 98% and iha, 26%), alpha-hemolysins ( hlyA, 10%), outer membrane protease ( ompT, 67%), aerobactin (iucD, 67%), iron-repressible protein ( irp2, 91%) and salmochelin ( iroN, 33%) and cytotoxic necrotizing factor 1 ( cnf1). Methods: Escherichia coli isolates (n = 94) were separated from the feces of patients attending teaching hospitals, and screened for various virulence genes: fimH, his, hlyA, ompT, irp2, iucD, iroN, and cnf1 by using the multiplex polymerase chain reaction (PCR) method. coli isolates collected from human feces in Zabol, southeast of Iran. coli strains, we determined the frequency of genes expressing virulence determinants in fecal E. To evaluate the health threats coordinated with a given fecal source of E. coli (FeEC) by the possession of certain virulence factors is able to cause diseases in human and other mammalian models. coli isolates causing urinary tract infections (UTIs). Objective: Escherichia coli is commensal bacterium of human intestine.